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Noa B. Martín-Cófreces / Francisco Sánchez-Madrid / José Ma
The Cytoskeleton
This volume explores techniques for studying, in detail, cytoskeletal structural and dynamic aspects that regulate cell organization and affect cell-cell interactions. The book features methods involving numerous areas of research, including the production of different relevant cytoskeletal proteins in native conditions, either in cells or in vitro, coupled to CCT chaperonin isolation for correct folding, the isolation of relevant organelles, actin and tubulin polymerization and motor transport, cryoelectron microscopy (CryoEM), post-translational modifications and protein localization, and more.