Springer Protocols

This volume provides readers with an overview of translational paths that can be used to study the pathophysiology of Parkinson’s Disease and atypical parkinsonism, including molecular processes, tissue integrity, and cerebral networks during inflammatory and neurodegenerative processes. The chapters in this book are organized into three parts and cover topics such as new developments of cerebral circuit characterizations and computational neuroscience for the description of the brain’s transition through disease stages; static and dynamic systemic changes with clinical and functional outcome measures; and atypical syndromes such as neurodegenerations, immunological processes, and progressive disease states. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Translational Methods for Parkinson’s Disease and Atypical Parkinsonism Research is a valuable resource that provides a new perspective on the disease from a scientific and clinical point of view, and highlights the importance of available and emerging technologies.

This detailed volume explores methods and protocols employed to investigate the multifaceted processes involved in plant stress memory. Opening with foundational methods for studying thermomemory in Arabidopsis thaliana, the book continues with techniques for studying memory mechanisms in other plant species, memory of biotic stressors, and the critical role of signaling molecules, such as jasmonic acid and salicylic acid. A key focus of this book is the investigation of DNA methylation and other epigenetic modifications that play a central role in the transmission of stress memory across generations, and epigenetic and transcriptomic approaches plus cutting-edge techniques such as RNA-Seq, CRISPR/Cas9-based gene editing, and proteomic analyses are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Stress Memory: Methods and Protocols serves as an invaluable resource for researchers, students, and practitioners working to unravel the mysteries of plant stress memory and apply this knowledge to address global challenges in agriculture and environmental sustainability.