Tissue Engineering and Regeneration

Cardiovascular diseases are still the leading cause of death in developed countries. Revascularization procedures such as coronary artery and peripheral bypass grafts, as well as access surgery represent a 2$ billion market yearly for the US alone. Despite intense research over many decades, no clinically suitable, shelf-ready, synthetic, vascular, small-caliber graft exists. There is therefore still a quest for such a clinical vascular prosthesis for surgical revascularization procedures and access surgery. Many approaches have been tried and are currently under investigation with promising results. These range from acellular and cell-based, stable or bio-degradable, synthetic scaffolds to biological or decellularized grafts, not forgetting self-assembly technologies for in vitro or in vivo VTE.  All these approaches can be further enhanced by functionalization, e.g. with growth factors and drug elution. This updatable book aims tocover all the relevant aspects of Vascular Tissue Engineering (VTE) and novel alternatives to develop vascular grafts for clinical applications. The chapters in this book cover different aspects of manufacturing scaffolds with various polymers, mechanical characteristics, degradation rates, decellularization techniques, cell sheet assembly, 3-D printing and autologous mandril-based VTE. All the necessary in vitro tests such as biocompatibility and thrombogenicity are reviewed. Pre-clinical assessment of in vivo experimental models include patency, compliance, intimal hyperplasia, inflammatory reaction, cellular ingrowth and remodeling. Finally, early clinical trials will be periodically updated regarding results, regulatory aspects and post-marketing quality assessment. Furthermore, the reader should get an insight into various approaches, technologies and methods to better understand the complexity of blood surface and cell interactions in VTE. Translational research has yielded early human applications clearly showing the enormous need of research in the field to provide better solutions for our patients and this continuously updated book will hopefully become a reference in the field for life sciences.

This reference work gives a comprehensive overview about how to fully exploit the potential of bioimaging to characterize tissue regeneration holistically and to gain complementary, multi-scale information about relevant structure, function, dynamics and molecular composition. To keep pace with the highly dynamic nature of the field and fast-developing imaging technology, it is essential to allow its Live Reference version to be updated and dynamic. It guides the reader through real-case biomedical applications organized by chapter and gives a tissue-specific overview on how to best assess parameters of interest. Additionally, all imaging techniques, including emerging modalities, are explained in great detail and enables the reader to understand the physical principles behind each imaging modality. The book provides a unique overview of available biological and (pre)clinical imaging techniques in the field and enables the reader to understand their benefits and limitations for real-case applications. The reader will be equipped with a comprehensive tool to properly chose the most promising technique for her/his biomedical research question - and respond to novel trends due to the dynamic nature of the Live Reference version available online. The book is of high interest to all researchers, physicians, physicists and life scientists working in the field to deepen their theoretical and practical knowledge about bioimaging and about the wide range of tissue-specific biomedical applications. The book is a valuable reference in the field for bioimaging.